RG Isotope analysis

The research group isotope analysis deals with the development and application of new analytical methods for isotope analysis. The research includes the development of new methods for analyte-matrix separation, the development of species-specific isotope measurements and the metrological implementation of isotope analysis.

The main focus of the analysis is on mass spectrometric methods ((MC) -IP-MS, TIMS). Another research focus on the further development of spectrometric methods (LAMIS, HRCSIRS) is planned.

The analyzed isotope systems in the main research areas include the systems Sr, S, Pb, Hg, Ca, Zn and B.

The applications cover the area of ??risk assessment of technological critical elements, provenance studies of environmental and geomaterials, as well as elemental cycling in biological systems and environmental systems.

Current projects

Abstract: New scientific knowledge to environmental geochemistry of conventional and modern inorganic pollutants will be obtained on a European river catchment on the example of the Mur/Mura River. Data acquired from this project will also serve as an expert knowledge baseline for potential improvement of regulatory framework for river waters and sediments, with an emphasis to emerging pollutants and technology-critical elements.

A holistic approach towards cross-border geochemical and isotopic tracking of the fluvial system and its tributaries will be carried out. The project will determine (1) natural geochemical background of the river’s catchment area, (2) the historic and recent anthropogenic sources of elements, (3) interaction between solid and liquid phases in different physical and chemical water conditions, (4) individual particles - carriers of specific pollutants, (5) differences in elemental composition of water and sediments in high, medium and low water regime (6) the potential contamination and baseline levels of emerging modern high-technology pollutants, (7) chemical and isotopic composition (based on XRF and MC ICP-MS) of drainage systems, including drainage waters and drainage sediments and will (8) establish a sampling, analytical and data curation protocols for such complex dataset. The obtained data and information will be (9) merged into easily understandable set of ecological indicators and maps.

  • Project funding: CEUS (ARRS-FWF) (International collaboration project SLOVENIA-AUSTRIA)
  • Project start: Early 2022
  • Project duration: 3 years

Team-MUL: Johanna Irrgeher (PI, AACH), Ulrike Moser (AACH), Daniel Vollprecht (AVAW), Thomas Prohaska (AACH)

Team-Cooperating institutions:
Slovenian Geological Survey GeoZS (PI: Dr. Gorazd Žibret, Dr. Polona Kralj, Dr. Robert Šajn, Dr. Miloš Miler, Dr. Meta Dobnikar, Dr. Klemen Teran, Emil Pučko, Mladen Štumergar, the National Institute of Chemistry (NIC) (Kristina Mervič, Dr. Martin Šala, Dr. Samo Hočevar) in Slovenia

Abstract: Chemistry should be for everyone, which includes accessibility and operability of analytical instrumentation, such as software but also hardware (e.g. mass spectrometers…)

Within this project, we aim at evaluating support strategies for promoting accessibility and operability of disabled scientists to analytical instrumentation. This includes a critical evaluation of barriers and difficulties currently in place. In close dialogue with disabled chemistry students and scientists and in cooperation with instrument manufacturers we would like to elaborate on possibilities on how to remove barriers to analytical instrumentation for operators with disabilities, e.g. including visually impaired individuals or persons using a wheelchair.

This may include changes of the instrument hardware and accessibility but also needs for software to be inclusive, modifying, adapting or rewriting laboratory protocols for the inclusion of existing assistive technologies, to enable chemists with disabilities of different nature to undertake laboratory experiments independently.

  • Project funding: Royal Society of Chemistry (RSC), Inclusion & Diversity Fund (https://www.rsc.org/prizes-funding/funding/inclusion-diversity-fund/)
  • Project start: January 2022

Webpage:  https://icpms-leoben2022/inclusion-in-lab

Team-MUL: Johanna Irrgeher (PI) & team

 

Abstract: Metal hyperaccumulation is a fascinating phenomenon. About 1% of higher plant species are able to accumulate 100-1000 times higher concentrations of trace elements than “normal” or nonaccumulating plant species in their aboveground tissues without showing any symptoms of toxicity. Most of these species accumulate nickel, whereas the accumulation of other elements (e.g. arsenic, cadmium, zinc) is much less frequent. While physiological and molecular processes have already been investigated in depth, Ni solubilisation processes in the rhizosphere are still largely unknown.

Novel rhizosphere research methods, including root exudate analysis from soil grown plants and highend isotope ratio analytics may offer new opportunities for gaining detailed insight into biogeochemical processes in the rhizosphere of Nickel hyperaccumulators.

This project aims to elucidate plant/microbe induced Nickel solubilisation mechanisms by assessing biogeochemical changes in the rhizosphere induced by roots and associated microorganisms. Isotope ratio analysis will be developed and implemented as a novel tool in rhizosphere research and applied for determining the major soil Ni pools acting as a source for plant-available nickel.

  • Project funding: FWF Austrian Science Fund
  • Project partners: The project is based at the University of Natural Resources and Life Sciences Vienna (BOKU Wien).
  • Project start: September 2021
  • Project end: August 2024

Team-MUL: Thomas Prohaska (PI), Alexander Epov, Stepan Chernonozhkin, Johanna Irrgeher

Team-Cooperating institutions: Markus Puschenreiter (PI-BOKU), Alice Tognacchini (BOKU), Olivier Donard (PAU, CNRS)

 

Interlaboratory Comparison – 87Sr/86Sr characterisation of cement, limestone and slate reference materials

Abstract: The type and origin of the construction materials and raw materials used, but also the manufacturers of the components, are of great relevance for damage prevention, for damage assessment, for the resulting liability issues, for forensic investigations, for restoring old buildings and when considering historical concretes. 87Sr/86Sr isotopic analysis has great potential for fingerprinting building materials, including cement and concrete. In light of this, the Bundesanstalt für Materialforschung und -prüfung (BAM) is organizing the interlaboratory comparison for the characterisation of 87Sr/86Sr isotope ratios in cement, limestone and slate reference materials by applying the conventional method for 87Sr/86Sr. The characterisation study is a part of a BAM project to establish an analytical procedure for cement provenancing. The Isotope Research Group participates in this interlaboratory comparison.

  • Project partners: Bundesanstalt für Materialforschung und -prüfung (BAM), Germany, supported by the International Association of Geoanalysts (IAG)
  • Project start: April 2021
  • Project end: (if applicable) December 2021

Team-MUL: Jochen Vogl (PI, BAM), Anera Kazlagic (BAM), Anika Retzmann, Johanna Irrgeher, Thomas Meisel, Thomas Prohaska, and further participants of the ILC

Metrology for the recycling of Technology Critical Elements to support Europe’s circular economy agenda

Abstract: Technology critical elements (TCEs) are vastly used throughout society; including phones, computers, and renewable energy products, such as solar panels and wind turbines. However, dwindling supplies of TCEs threaten to disrupt such technology production worldwide, which is especially concerning given a recent drive for more renewable energy sources. In 2017, the European Commission issued new targets for recycling TCE containing waste, however progress towards this goal is limited due to low availability of standards for quantification of these elements in urban mine waste matrices – a substantially challenging matrix due to the high heterogeneity of samples.

As such, the aim of this collaborative project is to develop new, SI traceable reference materials and methodology for accurate and precise quantification of TCEs in urban mine waste to aid with new recycling strategies. At the Chair of General and Analytical Chemistry, Montanuniversität Leoben, our primary aim is to explore the use of tandem ICP-mass spectrometry (ICP-MS/MS) and x-ray fluorescence spectrometry (XRF) measurement approaches for urban mine waste matrices. Consequently, new sample preparation strategies, including the use of pressurized microwave digestion, will also be developed. Additionally, laser ablation imaging will be used to assess the distribution of TCEs in the waste samples, ultimately assisting with the generation of a suitable new reference material.

  • Project funding: European Metrology Programme for Innovation and Research (EMPIR) and EU Horizon 2020.
  • Project partners: Laboratoire national de métrologie et d'essais (LNE), France. Bundesanstalt fuer Materialforschung und –pruefung (BAM), Germany. Institut za mjeriteljstvo Bosne i Hercegovine (IMBiH), Bosnia and Herzegovina. Istituto Nazionale di Ricerca Metrologica (INRIM), Italy. LGC Limited (LGC), United Kingdom. Physikalisch-Technische Bundesanstalt (PTB), Germany. RISE Research Institutes of Sweden AB (RISE), Sweden. Suomen ymparistokeskus (SKYE), Finland. Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK), Turkey. BRGM, France. ERAMET IDEAS (EID), France. Helmholtz-Zentrum Geesthacht Zentrum für Material- und Küstenforschung GmbH (HZG), Germany. Institut Jožef Stefan (JSI), Slovenia, Eidgenössisches Institut für Metrologie METAS (METAS), Switzerland.
  • Project start: June 2021
  • Project website:www.metrocycle.eu

Team-MUL: Johanna Irrgeher (PI), Melissa Eberhard, Shaun Lancaster, Alessandra Rachetti

 

TecEUS - Technology-Critical Elements in Urban Spheres

Abstract: The project aims at the systematic quantification of drivers, sources, pathways and sinks as well as environmental and human health threats of technology-critical elements (TCEs; e.g. tantalum, niobium, antimony, neodymium) in the urban spheres of Vienna. Within this project, TCE levels will be measured systematically in water, soil, plants and aerosol, supported by citizen science approaches involving residents and gardeners. Novel analytical techniques based on inductively-coupled plasma mass spectrometry (ICP-MS) will allow to detect the low levels of TCEs expected in natural samples. Green façades and plants will be evaluated for the efficiency to bind airborne TCEs both in field studies and in controlled wind channel box exposure experiments. A simplified socio-ecological model will be developed depicting the biophysical stocks and flows of TCEs and their human health implications along with the development of prospective scenarios and monitoring requirements. Maps of exposure and emissions will be made publicly available.

The transdisciplinary project combines analytical environmental chemistry (Montanuniversität Leoben, Dept. of Chemistry), landscape engineering (University of Natural Resources Vienna, Institute of Soil Bioengineering and Landscape Construction), socio-ecological modelling (University of Natural Resources Vienna, Institute of Social Economy) and human health (Medical University Vienna, Centre of Public Health).

  • Project funding: FWF Stand Alone Project
  • Project partners: University of Natural Resources and Life Sciences Vienna (BOKU), Medical University Vienna, TU Wien
  • Project start: April 2020 Project
  • Project website:  www.teceus.at

Publications: Spörl, P.; Göndör, A.;Irrgeher, J.; Prohaska, T.; Trimmel, S.;Capari, L.; Haluza, D.; Scharf, B.; Kasper-Giebl, A.; Pitha, U. Development of a Mobile Module-Based Wind Tunnel for the Determination of Collection Efficiencies of Particulate Matter on Surface Structures. Sustainability 2021, 13, 9565. https://doi.org/10.3390/su13179565

Team: Christine Opper, Donata Bandoniene, Thomas Prohaska, Johanna Irrgeher (PI) Laura Feiner, Maria Feiner, Nagi Lashin, Martina Maurer, Julia Retter, Simone Trimmel

 

Hydrochemical Characterisation of Deep Groundwater Systems in Upper Austria using multi-elemental fingerprints and Strontium Isotope Ratios

Abstract: The project coordinated by the Chair of Petroleum Geology aims at the source characterization of groundwater sources in Upper Austria based on multielemental pattern and strontium isotope ratio analysis. Within her Master thesis, Virginia Foelserl (Master Geology) uses multielemental data in combination with strontium isotope ratios assessed in a large sample set taken at different sampling stations in Upper Austria and assessed by (MC) ICP-MS to trace the lateral and vertical distribution of hydrostatigraphic units in the area.

  • Project start at AAC: October 2019
  • Project funding: ÖAW - Programm: Earth System Sciences (ESS

Team:Virginia Foelserl, Christine Opper, Johanna Irrgeher, Doris Groß(project PI, Chair of Petroleum Geology)

 

Elemental and isotopic fingerprinting of dust from historical artefacts

Abstract: The project is dedicated to the development and application of multielemental analysis along with isotope ratios to trace the sources of dust collected from historical artefacts in order to gain more information about authenticity and life history of highly precious objects.

  • Project funding: Third party funding
  • Project partners: Patricia Engel (Donau-Universität Krems, Austria)
  • Project start: January 2019

Project Team:Johanna Irrgeher, Christine Opper, Thomas Prohaska

 

Investigation of the fate of Pb in human systems

Abstract: Exposure to Pb can cause substantial organ damage. The concomitant intake of clinoptilolite tuff (zeolite) may reduce the enteral Pb absorption. In a medical study, enriched 204Pb isotopes were applied to monitor the fate of trace-level Pb in the human body. Pb isotope ratio measurements were performed using ICP-MS. Based on the natural isotopic composition prior to treatment with enriched 204Pb, isotope pattern deconvolution (IPD) was successfully applied as mathematical tool to monitor the fate of 204Pb and its reduced uptake due to clinoptilolite tuff in the human body.

  • Project funding: Third party funding
  • Project partners: Glock Health Gmbh
  • Project start: November 2018
  • Project end: (if applicable)
  • Project website:-

Publications: Samekova, K., Firbas, C., Irrgeher, J., Opper, C., Prohaska, T., Retzmann, A., . . . Wolzt, M. (2021). Concomitant oral intake of purified clinoptilolite tuff (G-PUR) reduces enteral lead uptake in healthy humans. Scientific Reports, 11(1), 14796. doi:10.1038/s41598-021-94245-x

Team-MUL: Johanna Irrgeher (PI), Christine Opper, Anika Retzmann, Thomas Prohaska

Team-Cooperating institutions: Gouya Insights, Medical University Vienna

 

Development of species-specific S isotope ratio measurements in biological material

Abstract: The project is dedicated to the development and optimization of a species-specific method for determining the isotopic composition of sulfur contained in the amino acids cysteine and methionine in hair samples. First results of the development of a suitable sample preparation method (hydrolysis of the proteins contained in the hair), the separation of cysteic acid and methionine sulfone by means of ion chromatography and the determination of the sulfur isotopic composition by means of MC ICP-MS will be further applied to a variety of biological systems.

  • Project funding: Third party funding
  • Project partners: University of Natural Resources and Life Sciences Vienna (AT), University of Calgary (CA)
  • Project start: October 2018

Project Team:Johanna Irrgeher (PI), Stephan Hann, Michael Schober, Hedda Drexler, Michael Wieser, Kerri Miller, Aaron Wilkins

 

Ca isotopes as tracers in life sciences

Abstract: Biological processes like biomineralization and the human Ca homeostasis produces significant fractionation of Ca isotopes. The analysis of Ca isotopes in biological tissue, including human bone, blood, urine and hair, has great potential to identify changes in Ca metabolism and bone metabolism, which are linked e.g. to increased bone resorption due to a disease like senile osteoporosis, multiple myeloma, kidney diseases, and diabetes. In this project, we developed a fully validated double-spike MC TIMS measurement procedure to determine low-amount Ca isotope ratios in limited biological samples. Further, for the first time Ca isotopic analysis of hair reference materials indicated a potential fractionation of Ca incorporated into hair tissue when compared to the blood pool. The laboratory work was contucted at the University of Calgary (Canada) in Professor Mike Wieser’s Isotope Lab.

  • Project funding: Chemical Monthly fellowship (2018) of the Austrian Academy of Sciences (ÖAW), Discovery Research Grant by the Natural Sciences and Engineering Research Council of Canada (NSERC), Faculty of Science Grand Challenges Fund of the University of Calgary.
  • Project partners: University of Calgary, Department of Physics and Astronomy, Stable Isotope Laboratory, Canada
  • Project start: January 2019

Publications: Retzmann, A., Walls, D., Miller, K., Wieser, M., Irrgeher, J., & Prohask, T. (2021). Assessing the potential of online ICP–MS analysis to optimize Ca/ matrix separation using DGA Resin for subsequent isotopic analysis. Monatshefte für Chemie, 152, 401-410. doi:10.1007/s00706-021-02754-2

Retzmann, A., Walls, D., Miller, K. A., Irrgeher, J., Prohaska, P., Wieser, M. E. (2021). A double-spike MC TIMS measurement procedure for low-amount Ca isotopic analysis of limited biological samples. Analytical and Bioanalytical Chemistry. doi: 10.1007/s00216-021-03650-8

Team-MUL: Anika Retzmann, Michael E. Wieser, Dorothy Walls, Kerri A. Miller, Johanna Irrgeher, Thomas Prohaska

 

Mammoth Migration in Alaska

Abstract: Within a wide-scope project hosted at the University of Fairbanks (USA) dedicated to a better understand why woolly mammoths survived late into the mid-Holocene only in the environments of arctic islands of the Bering Land Bridge. Furthermore, this research is testing various hypotheses proposed to explain the extinction of the Holocene mammoth population on St. Paul Island, Pribilof Islands, Alaska, as well as establish the actual time of extinction. We are happy to contribute to the development of analytical methods dedicated to Sr isotope ratios based on LA-MC ICP-MS.

  • Project partners: University of Alaska Fairbanks (USA), University of Calgary (CA)
  • Project start: June 2018

Learn more:http://ine.uaf.edu/werc/people/faculty/mat-wooller/